Chromatofocusing purification of CD1b-antigen complexes and their analysis by isoelectric focusing.

Luis Fernando Garcia-Alles, Henri de la Salle

Institut de Pharmacologie et de Biologie Structurale (IPBS), UMR 5089 CNRS/Université Toulouse III-Paul Sabatier, Toulouse, France. luis-fernando.garcia-alles@ipbs.fr

Methods in molecular biology (Clifton, N.J.) 2013

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The presentation of lipid antigens to T cells is mediated by the CD1 proteins. Purified functional CD1/lipid complexes are valuable tools to investigate such immune processes. Here, we describe how these complexes can be prepared in vitro, how they can be purified by chromatofocusing and how to control their antigen-loading status by isoelectric focusing.

Citation

Luis Fernando Garcia-Alles, Henri de la Salle. Chromatofocusing purification of CD1b-antigen complexes and their analysis by isoelectric focusing. Methods in molecular biology (Clifton, N.J.). 2013;960:573-83