FRET excited ratiometric oxygen sensing in living tissue.

Justin M Ingram, Chunfeng Zhang, Jian Xu, Steven J Schiff

Journal of neuroscience methods 2013 Mar 30

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Dynamic analysis of oxygen (O₂) has been limited by the lack of a real-time, quantitative, and biocompatible sensor. To address these demands, we designed a ratiometric optode matrix consisting of the phosphorescence quenching dye platinum (II) octaethylporphine ketone (PtOEPK) and nanocystal quantum dots (NQDs), which when embedded within an inert polymer matrix allows long-term pre-designed excitation through fluorescence resonance energy transfer (FRET). Depositing this matrix on various glass substrates allowed the development of a series of optical sensors able to measure interstitial oxygen concentration [O₂] with several hundred millisecond temporal resolution in varying biological microdomains of active brain tissue. Published by Elsevier B.V.

Citation

Justin M Ingram, Chunfeng Zhang, Jian Xu, Steven J Schiff. FRET excited ratiometric oxygen sensing in living tissue. Journal of neuroscience methods. 2013 Mar 30;214(1):45-51