Lymphocyte glucocorticoid receptor expression level and hormone-binding properties differ between war trauma-exposed men with and without PTSD.

University of Belgrade, Institute for Biological Research Siniša Stanković, Department of Biochemistry, Belgrade, Serbia. gormatic@ibiss.bg.ac.rs

Progress in neuro-psychopharmacology & biological psychiatry 2013 Jun 3

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Posttraumatic stress disorder (PTSD) has been shown to be associated with altered glucocorticoid receptor (GR) activity. We studied the expression and functional properties of the receptor in peripheral blood mononuclear cells (PBMCs) from non-traumatized healthy individuals (healthy controls; n=85), and war trauma-exposed individuals with current PTSD (n=113), with life-time PTSD (n=61) and without PTSD (trauma controls; n=88). The aim of the study was to distinguish the receptor alterations related to PTSD from those related to trauma itself or to resilience to PTSD. Functional status of the receptor was assessed by radioligand binding and lysozyme synthesis inhibition assays. The level of GR gene expression was measured by quantitative PCR and immunoblotting. Current PTSD patients had the lowest, while trauma controls had the highest number of glucocorticoid binding sites (Bmax) in PBMCs. Hormone-binding potential (Bmax/KD ratio) of the receptor was diminished in the current PTSD group in comparison to all other study groups. Correlation between Bmax and KD that normally exists in healthy individuals was decreased in the current PTSD group. Contrasting Bmax data, GR protein level was lower in trauma controls than in participants with current or life-time PTSD. Current PTSD is characterized by reduced lymphocyte GR hormone-binding potential and by disturbed compensation between Bmax and hormone-binding affinity. Resilience to PTSD is associated with enlarged fraction of the receptor molecules capable of hormone binding, within the total receptor molecule population in PBMCs. Copyright © 2013 Elsevier Inc. All rights reserved.

Citation

Gordana Matić, Danijela Vojnović Milutinović, Jelena Nestorov, Ivana Elaković, Sanja Manitašević Jovanović, Tatjana Perišić, Jadranka Dunđerski, Svetozar Damjanović, Goran Knežević, Željko Špirić, Eric Vermetten, Danka Savić. Lymphocyte glucocorticoid receptor expression level and hormone-binding properties differ between war trauma-exposed men with and without PTSD. Progress in neuro-psychopharmacology & biological psychiatry. 2013 Jun 3;43:238-45