Metabolic profiles of Nannochloropsis oceanica IMET1 under nitrogen-deficiency stress.
Yan Xiao, Jingtao Zhang, Jiatao Cui, Yingang Feng, Qiu Cui
Shandong Provincial Key Laboratory of Energy Genetics, Key Laboratory of Biofuels, Qingdao Institute of Bioenergy and Bioprocess Technology, Chinese Academy of Sciences, 189 Songling Road, Qingdao, Shandong 266101, PR China.
Bioresource technology 2013 FebTo understand the mechanism of lipid accumulation and the corresponding metabolic changes of the microalga Nannochloropsis oceanica IMET1, the lipid content, fatty acid composition and metabolic profile were investigated via batch culture under nutrient deficiency and chemostatic culture under nitrate limitation. The results indicated that the triacylglycerol-neutral lipids were significantly accumulated through an acyl-CoA dependent pathway, while the polar lipids were partially converted to triacylglycerol through an acyl-CoA independent pathway. The fatty acid compositions of the polar lipids changed concurrently with the length of time of the nutrient deficiency, while the fatty acid compositions of the neutral lipids remained nearly consistent. The concentrations of several major osmolytes were significantly changed under chemostatic conditions with different nitrogen concentrations, which reflect the membrane property changes caused by the alteration of the polar lipid composition. Copyright © 2012 Elsevier Ltd. All rights reserved.
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Yan Xiao, Jingtao Zhang, Jiatao Cui, Yingang Feng, Qiu Cui. Metabolic profiles of Nannochloropsis oceanica IMET1 under nitrogen-deficiency stress. Bioresource technology. 2013 Feb;130:731-8
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PMID: 23334034
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