Chemiluminescence determination of folic acid by a flow injection analysis assembly.

Saikh Mohammad Wabaidur, Seikh Mafiz Alam, Sang Hak Lee, Zeid Abdullah Alothman, Gaber E Eldesoky

Advanced Materials Research Chair, College of Science, King Saud University, Riyadh 11451, Saudi Arabia. tarabai22@yahoo.com.sg

Spectrochimica acta. Part A, Molecular and biomolecular spectroscopy 2013 Mar 15

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A flow injection (FI) method is reported for the determination of folic acid by chemiluminescence method. This method is based on the reaction of folic acid with Ru(bipy)(3)(2+) and Ce(IV) to produce chemiluminescence. The calibration curve was linear over the range of 2.5×10(-5)-3.1×10(-7) mol/L with a detection limit of 2.3×10(-8) mol/L (S/N=3). The relative standard deviation of 1.0×10(-6) mol/L folic acid was found 3.5% (n=11). The influences of potential interfering substances were studied. The recovery was higher than 95.3%. The method was accurate, sensitive, and effective for assay of folic acid. This CL method was successfully applied to the determination of folic acid in pharmaceutical preparations. The mechanism of CL reaction was also studied. Copyright © 2012 Elsevier B.V. All rights reserved.

Citation

Saikh Mohammad Wabaidur, Seikh Mafiz Alam, Sang Hak Lee, Zeid Abdullah Alothman, Gaber E Eldesoky. Chemiluminescence determination of folic acid by a flow injection analysis assembly. Spectrochimica acta. Part A, Molecular and biomolecular spectroscopy. 2013 Mar 15;105:412-7