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SP1 protein as a new type of biological nanopore is described and is utilized to distinguish single-stranded DNA at the single-molecule level. Using the SP1 nanopore to investigate single molecule detection broadens the existing research areas of pore-forming biomaterials from unsymmetrical biological nanopores to symmetrical biological nanopores. This novel nanopore could provide a good candidate for single-molecule detection and characterization of biomaterial applications.


Hai-Yan Wang, Yang Li, Li-Xia Qin, Arnon Heyman, Oded Shoseyov, Itamar Willner, Yi-Tao Long, He Tian. Single-molecule DNA detection using a novel SP1 protein nanopore. Chemical communications (Cambridge, England). 2013 Feb 28;49(17):1741-3

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PMID: 23340583

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