Fast microwave-assisted extraction of rotenone for its quantification in seeds of yam bean (Pachyrhizus sp.).

Emmanuelle Lautié, Catherine Rasse, Eric Rozet, Claire Mourgues, Jean-Paul Vanhelleputte, Joëlle Quetin-Leclercq

Laboratoire de Pharmacognosie, LDRI, Université Catholique de Louvain, Brussels, Belgium. emmanuelle.lautie@uclouvain.be

Journal of separation science 2013 Feb

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The aim of this study was to find if fast microwave-assisted extraction could be an alternative to the conventional Soxhlet extraction for the quantification of rotenone in yam bean seeds by SPE and HPLC-UV. For this purpose, an experimental design was used to determine the optimal conditions of the microwave extraction. Then the values of the quantification on three accessions from two different species of yam bean seeds were compared using the two different kinds of extraction. A microwave extraction of 11 min at 55°C using methanol/dichloromethane (50:50) allowed rotenone extraction either equivalently or more efficiently than the 8-h-Soxhlet extraction method and was less sensitive to moisture content. The selectivity, precision, trueness, accuracy, and limit of quantification of the method with microwave extraction were also demonstrated. © 2013 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.

Citation

Emmanuelle Lautié, Catherine Rasse, Eric Rozet, Claire Mourgues, Jean-Paul Vanhelleputte, Joëlle Quetin-Leclercq. Fast microwave-assisted extraction of rotenone for its quantification in seeds of yam bean (Pachyrhizus sp.). Journal of separation science. 2013 Feb;36(4):758-63