Characteristics of neutralizing antibodies to adenovirus capsid proteins in human and animal sera.

Bin Yu, Jianing Dong, Chu Wang, Yang Zhan, Haihong Zhang, Jiaxin Wu, Wei Kong, Xianghui Yu

National Engineering Laboratory for AIDS Vaccine, College of Life Science, Jilin University, Changchun 130012, China.

Virology 2013 Mar 15

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Although it is known that Ad5-specific neutralizing antibodies (NAbs) against three major viral capsid components (hexon, penton and fiber) are generated, differences in the frequency and nature of these pre-existing NAbs remain unclear. The results emphasized the contribution of anti-fiber antibodies to Ad5 neutralization responses generated during natural viral infection. Additionally, Ad5-specific NAbs against the fiber knob protein were present in over 90% of the positive serum samples while 42% of the sera had NAbs against hexon in this study based on neutralization assay of anti-HVR and anti-knob subtracted sera and Western blotting analysis. We also found that the trimeric knob was preferentially recognized by fiber-induced NAbs and it was serotype-specific in human adenovirus species C. Results indicated that the trimeric knob protein would be a good candidate antigen for detecting adenovirus serotype-specific NAbs in naturally infected sera. Copyright © 2013 Elsevier Inc. All rights reserved.

Citation

Bin Yu, Jianing Dong, Chu Wang, Yang Zhan, Haihong Zhang, Jiaxin Wu, Wei Kong, Xianghui Yu. Characteristics of neutralizing antibodies to adenovirus capsid proteins in human and animal sera. Virology. 2013 Mar 15;437(2):118-23