A rhodamine based fluorescent probe for Hg2+ and its application to cellular imaging.

Fanyong Yan, Donglei Cao, Ning Yang, Meng Wang, Linfeng Dai, Chuying Li, Li Chen

State Key Laboratory of Hollow Fiber Membrane Materials and Processes, Key Lab of Fiber Modification & Functional Fiber of Tianjin, Tianjin Polytechnic University, Binshui West Road 399, Tianjin 300387, PR China.

Spectrochimica acta. Part A, Molecular and biomolecular spectroscopy 2013 Apr

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A new rhodamine-based fluorescent probe (Rh-F) for detection of Hg(2+) ions was synthesized, which could bind Hg(2+) in aqueous ethanol (7:3, v/v) at pH 7.0 with detectable change in color and fluorescence. The response is based on a ring opening reaction and formation of a 1:1 complex, while ring-opening process of spirolactam enables large fluorescent enhancement and colorimetric change upon the addition of Hg(2+). The response is reversible and linear in the range between 200nM and 1000nM, with a detection limit of 4.2nM. Selectivity and competition experiments with various other metal ion revealed that Rh-F possesses highly selective fluorescent response to Hg(2+). Furthermore, the probe was successfully applied to fluorescent imaging of Hg(2+) in L-929 cells confirm that Rh-F can be used as a fluorescent probe for monitoring Hg(2+) in living cells. Copyright © 2013 Elsevier B.V. All rights reserved.

Citation

Fanyong Yan, Donglei Cao, Ning Yang, Meng Wang, Linfeng Dai, Chuying Li, Li Chen. A rhodamine based fluorescent probe for Hg2+ and its application to cellular imaging. Spectrochimica acta. Part A, Molecular and biomolecular spectroscopy. 2013 Apr;106:19-24