Niche-less maintenance of HSCs by 2i.

Shigeo Masuda, Mo Li, Juan Carlos Izpisua Belmonte

Gene Expression Laboratory, Salk Institute for Biological Studies, La Jolla, CA 92037, USA.

Cell research 2013 Apr

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Maintenance of hematopoietic stem cells (HSCs) in vitro has been believed to be difficult due to a lack of complete understanding of HSC quiescence maintained by the niche. Recent evidence suggests that in vitro maintenance of human and mouse long-term HSCs (LT-HSCs) is possible through dual inhibition (2i) of both GSK-3 and mTOR in the absence of cytokines, serum, or feeder cells.