Surveillance of Japanese encephalitis virus infection in mosquitoes in Vietnam from 2006 to 2008.

The American journal of tropical medicine and hygiene 2013 Apr

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Japanese encephalitis virus (JEV) infection in mosquitoes was monitored in Vietnam from 2006 to 2008. A total of 15,225 mosquitoes, identified as 26 species in five genera were collected and 12,621 were grouped into 447 pools for examination of JEV infection by assays for cytopathic effects in C6/36 cells and by RT-PCR to detect flavivirus RNA. Three JEV strains were isolated from Culex tritaeniorhynchus Giles collected in northern and southern Vietnam and two JEV strains were isolated from Culex vishnui Theobald collected in the highlands of Vietnam. Genetic and phylogenetic analyses, based on complete E gene nucleotide sequences, revealed that the five JEV strains were classified into the genotype I group and six amino acid differences were found in these five strains. These results indicated that multiple JEV genotype I populations are circulating countrywide in Vietnam, transmitted by bites of their Cx. tritaeniorhynchus and Cx. vishnui.

Citation

Ryusei Kuwata, Phan Thi Nga, Nguyen Thi Yen, Keita Hoshino, Haruhiko Isawa, Yukiko Higa, Nguyen Vet Hoang, Bui Minh Trang, Do Phuong Loan, Tran Vu Phong, Toshinori Sasaki, Yoshio Tsuda, Mutsuo Kobayashi, Kyoko Sawabe, Masahiro Takagi. Surveillance of Japanese encephalitis virus infection in mosquitoes in Vietnam from 2006 to 2008. The American journal of tropical medicine and hygiene. 2013 Apr;88(4):681-8