Rapid detection of several foodborne pathogens by F0F1-ATPase molecular motor biosensor.

Jie Zhang, Zhaojie Li, Huiyuan Zhang, Jing Wang, Yan Liu, Guangquan Chen

Beijing Entry-Exit Inspection and Quarantine Bureau, Beijing, China.

Journal of microbiological methods 2013 Apr

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F0F1-ATPase within chromatophore was constructed as a molecular motor biosensor through ε-subunit antibody-biotin-streptavidin-biotin-AC5-Sulfo-Osu system. Based on probe-DNA specific binding, DNA of several foodborne pathogens Listeria monocytogenes, Salmonella typhimurium, Vibrio parahaemolyticus and Vibrio cholerae was specifically captured by F0F1-ATPase molecular motor biosensors. Loads of DNA decreased the rotation rate of F0F1-ATPase, and led to the decrease of ATP synthesis. The detection of pathogens based on proton flux change driven by ATP-synthesis of F0F1-ATPase, which was indicated by F-DHPE, was monitored by a fluorescence spectrometer. The results demonstrate that the F0F1-ATPase molecular motor biosensor can specifically detect bacterial DNA at low concentration level, and will be a convenient, quick, and promising tool for detecting pathogens. Copyright © 2013 Elsevier B.V. All rights reserved.

Citation

Jie Zhang, Zhaojie Li, Huiyuan Zhang, Jing Wang, Yan Liu, Guangquan Chen. Rapid detection of several foodborne pathogens by F0F1-ATPase molecular motor biosensor. Journal of microbiological methods. 2013 Apr;93(1):37-41