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In vitro methods did not accurately predict in vivo apparent total tract digestibility of energy for canola coproducts (r = -0.81; P = 0.002). We scanned 153 samples of digesta, feces, and in vitro digestion residues (ivR) on a Fourier transform midinfrared instrument with a single-reflection attenuated total reflectance attachment. The second derivative spectral net intensities of the carbonyl ester peak at 1745 cm(-1) and olefinic hydrocarbon (=C-H) peak at 3008 cm(-1) were both higher in ivR than in feces (3.83 × 10(-04) vs. 3.46 × 10(-05) and 7.92 × 10(-05) vs. 5.17 × 10(-06), respectively; P < 0.001), indicating poor enzymatic digestion of unsaturated fat. In conclusion, fat digestion of in vitro procedure for canola coproducts requires improvement to adequately mimic in vivo digestion in pigs.

Citation

L F Wang, M L Swift, R T Zijlstra. Evaluation of energy digestibility of canola coproducts by in vitro analyses and characterization of fat digestion using spectroscopy. Journal of animal science. 2012 Dec;90 Suppl 4:251-3

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PMID: 23365346

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