A low-cost intracellular delivery system based on microbubble and high gravity field.

Chuan He, Quanrong Gu, Min Huang, Xiaoyan Yang, James Xing, Jie Chen

Department of Electrical and Computer Engineering, University of Alberta, Edmonton, Alberta, Canada.

Conference proceedings : ... Annual International Conference of the IEEE Engineering in Medicine and Biology Society. IEEE Engineering in Medicine and Biology Society. Conference 2012

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In this paper, we developed a low-cost intracellular delivery system based on microbubble and high gravity field. We successfully delivered FITC-Dextran (40kD) into hard-to-deliver THP-1 cells. The results showed that our method achieved high delivery efficiency up to 80%. It was found that the delivery efficiency and cell viability were closely related to the centrifuge speed. We speculated that the burst of microbubbles causes transient pore opening thus increasing the chance of biomolecules entering cells. This fast, low-cost and easy-to-operate protocol is very promising for delivering therapeutic genes and drugs into any cells which do not actively take up extracellular materials. This method is most effective for in-vitro delivery, but after delivery, treated cells might be injected back to human for in-vivo imaging.

Citation

Chuan He, Quanrong Gu, Min Huang, Xiaoyan Yang, James Xing, Jie Chen. A low-cost intracellular delivery system based on microbubble and high gravity field. Conference proceedings : ... Annual International Conference of the IEEE Engineering in Medicine and Biology Society. IEEE Engineering in Medicine and Biology Society. Conference. 2012;2012:2424-7