Viable circulating tumor cell enrichment by flexible micro spring array.

Ramdane A Harouaka, Ming-Da Zhou, Tim Y Yeh, Waleed J Khan, Jeffery Allerton, Si-Yang Zheng

Department of Bioengineering and Material Research Institute, The Pennsylvania State University, University Park, PA 16802, USA.

Conference proceedings : ... Annual International Conference of the IEEE Engineering in Medicine and Biology Society. IEEE Engineering in Medicine and Biology Society. Conference 2012

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We demonstrated a high throughput versatile platform capable of isolating circulating tumor cells (CTCs) from clinically relevant volumes of blood while preserving their viability and ability to proliferate. The enrichment is based on the fact that CTCs are larger compared with normal blood cells. The incorporated system allows size-based separation of CTCs at the micro-scale, while taking advantage of a high throughput and rapid processing speed. Testing results of model systems using cell lines show that this device can enrich CTCs from 7.5 mL of whole blood samples with 90% capture efficiency, higher than 10(4) enrichment, and better than 80% viability in approximately ten minutes without any incidence of clogging.

Citation

Ramdane A Harouaka, Ming-Da Zhou, Tim Y Yeh, Waleed J Khan, Jeffery Allerton, Si-Yang Zheng. Viable circulating tumor cell enrichment by flexible micro spring array. Conference proceedings : ... Annual International Conference of the IEEE Engineering in Medicine and Biology Society. IEEE Engineering in Medicine and Biology Society. Conference. 2012;2012:6269-72