Transformation of Candida guilliermondii wild-type strains using the Staphylococcus aureus MRSA 252 ble gene as a phleomycin-resistant marker.

Emilien Foureau, Vincent Courdavault, Andrew J Simkin, Andriy A Sibirny, Joël Crèche, Nathalie Giglioli-Guivarc'h, Marc Clastre, Nicolas Papon

EA2106, Biomolécules et Biotechnologies Végétales, Faculté de Pharmacie, Université François-Rabelais de Tours, Tours, France.

FEMS yeast research 2013 May

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We designed an efficient transformation system for Candida guilliermondii wild-type strains. We demonstrated that the Staphylococcus aureus MRSA 252 ble coding sequence placed under the control of the yeast phosphoglycerate kinase gene transcription-regulating regions confers phleomycin resistance to transformed C. guilliermondii cells. To illustrate the potential of this drug-resistant cassette, we carried out the disruption of the C. guilliermondii ADE2 gene. This new dominant selectable marker represents a powerful tool to study the function of various genes in this yeast of clinical and biotechnological interest. © 2013 Federation of European Microbiological Societies. Published by Blackwell Publishing Ltd. All rights reserved.

Citation

Emilien Foureau, Vincent Courdavault, Andrew J Simkin, Andriy A Sibirny, Joël Crèche, Nathalie Giglioli-Guivarc'h, Marc Clastre, Nicolas Papon. Transformation of Candida guilliermondii wild-type strains using the Staphylococcus aureus MRSA 252 ble gene as a phleomycin-resistant marker. FEMS yeast research. 2013 May;13(3):354-8