Correlation Engine 2.0
Clear Search sequence regions


Sizes of these terms reflect their relevance to your search.

This unit contains basic protocols for determining the conformational stability of a globular protein from either urea or thermal unfolding curves. Circular dichroism is the optical spectroscopic technique most commonly used to monitor protein unfolding. The protocols describe how to analyze data from an unfolding curve to obtain the thermodynamic parameters necessary to calculate conformational stability, and how to determine differences in stability between protein variants.

Citation

Gerald R Grimsley, Saul R Trevino, Richard L Thurlkill, J Martin Scholtz. Determining the conformational stability of a protein from urea and thermal unfolding curves. Current protocols in protein science / editorial board, John E. Coligan ... [et al.]. 2013;Chapter 28:Unit28.4

Expand section icon Mesh Tags

Expand section icon Substances


PMID: 23377851

View Full Text