CD4(+) T-cells are unable to express the HIV natural resistance factor globotriosylceramide.
Minji Kim, Beth Binnington, Darinka Sakac, Clifford A Lingwood, Donald R Branch
aDepartment of Medicine, University of Toronto bMolecular Structure and Function, Hospital for Sick Children Research Institute cCanadian Blood Services, Research and Development dDepartments of Laboratory Medicine and Pathobiology, and Biochemistry, University of Toronto, Toronto, Ontario M5G 2M1, Canada.
AIDS (London, England) 2013 Mar 27Globotriaosylceramide (Gb(3)) is a cell surface-expressed natural resistance factor for HIV infection, but, its expression in human T-cells remains unknown. Therefore, Gb(3) in resting or activated CD4(+) T-cells was assessed by flow cytometry and thin layer chromatography of cell extracts. We found the majority of CD4(+) T-cells, whether resting or activated, do not express Gb(3) at significant levels (<2% positive cells). Thus, HIV treatment or prevention strategies must focus on development of soluble Gb(3) analogues for inhibition of HIV infection.
Citation
Minji Kim, Beth Binnington, Darinka Sakac, Clifford A Lingwood, Donald R Branch. CD4(+) T-cells are unable to express the HIV natural resistance factor globotriosylceramide. AIDS (London, England). 2013 Mar 27;27(6):1029-32
PMID: 23380967
View Full Text
