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Virus-induced gene silencing in the rapid cycling columbine Aquilegia coerulea "Origami".
Department of Organismic and Evolutionary Biology, Harvard University, Cambridge, MA, USA.
Methods in molecular biology (Clifton, N.J.) 2013Sizes of these terms reflect their relevance to your search.
Aquilegia Origami is an emerging model system for ecology and evolution, which has numerous genetic and genomic tools. Virus-induced gene silencing (VIGS) has been established as an effective approach to study gene function in Aquilegia. In the current protocol, we demonstrate VIGS using Agrobacterium strain GV3101 carrying tobacco rattle virus (TRV)-based constructs to infect Aquilegia coerulea "Origami" plants via vacuum infiltration.
Citation
Bharti Sharma, Elena M Kramer. Virus-induced gene silencing in the rapid cycling columbine Aquilegia coerulea "Origami". Methods in molecular biology (Clifton, N.J.). 2013;975:71-81
Mesh Tags
PMID: 23386296
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