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We report a simplified micropatterning method for the straight-line extension of the neurites of cultured neurons. We prepared a poly-D-lysine (PDL)-patterned surface using a polydimethylsiloxane microfluidic stamp. Hippocampal neurons were cultured on the PDL-bound substrate with the stamp removed, allowing for conventional cell seeding and detailed optical observation without fluorescent label. Cultured neurons elongated neurites along straight lines at the single-cell level and displayed spontaneous firing as detected by time-lapse imaging and Ca(2+) imaging.

Citation

Ikuro Suzuki, Kosuke Nakamura, Aoi Odawara, Amani Alhebshi, Masao Gotoh. A simplified micropatterning method for straight-line neurite extension of cultured hippocampal neurons. Analytical sciences : the international journal of the Japan Society for Analytical Chemistry. 2013;29(2):263-6

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PMID: 23400294

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