Gene expression profile of highly purified bone marrow mast cells in systemic mastocytosis.

Servicio General de Citometría, Centro de Investigación del Cáncer (IBMCC-CSIC/USAL and IBSAL) and Departamento de Medicina, Universidad de Salamanca, Salamanca, Spain.

The Journal of allergy and clinical immunology 2013 Apr

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Despite the fact that a great majority (>90%) of patients with systemic mastocytosis (SM) carry a common genetic lesion, the D816V KIT mutation, little is known regarding the molecular and biological pathways underlying the clinical heterogeneity of the disease. We sought to analyze the gene expression profile (GEP) of bone marrow mast cells (BMMCs) in patients with SM and its association with distinct clinical variants of the disease. GEP analyses were performed by using DNA-oligonucleotide microarrays in highly purified BMMCs from patients with SM carrying the D816V KIT mutation (n=26) classified according to the diagnostic subtype of SM versus normal/reactive BMMCs (n=7). Validation of GEP results was performed with flow cytometry in the same set of samples and in an independent cohort of 176 subjects. Overall, 758 transcripts were significantly deregulated in patients with SM, with a common GEP (n=398 genes) for all subvariants of SM analyzed. These were characterized by upregulation of genes involved in the innate and inflammatory immune response, including interferon-induced genes and genes involved in cellular responses to viral antigens, together with complement inhibitory molecules and genes involved in lipid metabolism and protein processing. Interestingly, aggressive SM additionally showed deregulation of apoptosis and cell cycle-related genes, whereas patients with indolent SM displayed increased expression of adhesion-related molecules. BMMCs from patients with different clinical subtypes of SM display distinct GEPs, which might reflect new targetable pathways involved in the pathogenesis of the disease. Copyright © 2013 American Academy of Allergy, Asthma & Immunology. Published by Mosby, Inc. All rights reserved.

Citation

Cristina Teodosio, Andrés C García-Montero, María Jara-Acevedo, Laura Sánchez-Muñoz, Carlos E Pedreira, Ivan Álvarez-Twose, Sergio Matarraz, José M Morgado, Paloma Bárcena, Almudena Matito, Andrea Mayado, Maria Luz Sanchez, María Diez-Campelo, Luis Escribano, Alberto Orfao. Gene expression profile of highly purified bone marrow mast cells in systemic mastocytosis. The Journal of allergy and clinical immunology. 2013 Apr;131(4):1213-24, 1224.e1-4