Signal recognition initiates reorganization of the presequence translocase during protein import.

Oleksandr Lytovchenko, Jonathan Melin, Christian Schulz, Markus Kilisch, Dana P Hutu, Peter Rehling

The EMBO journal 2013 Mar 20

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The mitochondrial presequence translocase interacts with presequence-containing precursors at the intermembrane space (IMS) side of the inner membrane to mediate their translocation into the matrix. Little is known as too how these matrix-targeting signals activate the translocase in order to initiate precursor transport. Therefore, we analysed how signal recognition by the presequence translocase initiates reorganization among Tim-proteins during import. Our analyses revealed that the presequence receptor Tim50 interacts with Tim21 in a signal-sensitive manner in a process that involves the IMS-domain of the Tim23 channel. The signal-driven release of Tim21 from Tim50 promotes recruitment of Pam17 and thus triggers formation of the motor-associated form of the TIM23 complex required for matrix transport.

Citation

Oleksandr Lytovchenko, Jonathan Melin, Christian Schulz, Markus Kilisch, Dana P Hutu, Peter Rehling. Signal recognition initiates reorganization of the presequence translocase during protein import. The EMBO journal. 2013 Mar 20;32(6):886-98