Surface plasmon resonance for measuring interactions of proteins with lipid membranes.

Vesna Hodnik, Gregor Anderluh

Department of Biology, Biotechnical Faculty, University of Ljubljana, Ljubljana, Slovenia.

Methods in molecular biology (Clifton, N.J.) 2013

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Surface plasmon resonance (SPR) is an established method for studying molecular interactions in real time. It allows obtaining qualitative and quantitative data on interactions of proteins with lipid memb ranes. In most of the approaches, a lipid membrane or a membrane-mimetic surface is prepared on the surface of Biacore (GE Healthcare) sensor chips HPA or L1, and the studied protein is then injected across the surface. Here, we provide an overview of SPR in protein-membrane interactions, different approaches described in the literature, and a general protocol for conducting an SPR experiment including lipid membranes, together with some experimental considerations.

Citation

Vesna Hodnik, Gregor Anderluh. Surface plasmon resonance for measuring interactions of proteins with lipid membranes. Methods in molecular biology (Clifton, N.J.). 2013;974:23-36