Structure-based discovery of pyrazolobenzothiazine derivatives as inhibitors of hepatitis C virus replication.

Journal of medicinal chemistry 2013 Mar 28

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The NS5B RNA-dependent RNA polymerase is an attractive target for the development of novel and selective inhibitors of hepatitis C virus replication. To identify novel structural hits as anti-HCV agents, we performed structure-based virtual screening of our in-house library followed by rational drug design, organic synthesis, and biological testing. These studies led to the identification of pyrazolobenzothiazine scaffold as a suitable template for obtaining novel anti-HCV agents targeting the NS5B polymerase. The best compound of this series was the meta-fluoro-N-1-phenyl pyrazolobenzothiazine derivative 4a, which exhibited an EC50 = 3.6 μM, EC90 = 25.6 μM, and CC50 > 180 μM in the Huh 9-13 replicon system, thus providing a good starting point for further hit evolution.

Citation

Maria Letizia Barreca, Giuseppe Manfroni, Pieter Leyssen, Johan Winquist, Neerja Kaushik-Basu, Jan Paeshuyse, Ramalingam Krishnan, Nunzio Iraci, Stefano Sabatini, Oriana Tabarrini, Amartya Basu, U Helena Danielson, Johan Neyts, Violetta Cecchetti. Structure-based discovery of pyrazolobenzothiazine derivatives as inhibitors of hepatitis C virus replication. Journal of medicinal chemistry. 2013 Mar 28;56(6):2270-82