Single-chromosome transcriptional profiling reveals chromosomal gene expression regulation.

Marshall J Levesque, Arjun Raj

Nature methods 2013 Mar

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We report intron chromosomal expression FISH (iceFISH), a multiplex imaging method for measuring gene expression and chromosome structure simultaneously on single chromosomes. We find substantial differences in transcriptional frequency between genes on a translocated chromosome and the same genes in their normal chromosomal context in the same cell. Correlations between genes on a single chromosome pointed toward a cis chromosome-level transcriptional interaction spanning 14.3 megabases.

Citation

Marshall J Levesque, Arjun Raj. Single-chromosome transcriptional profiling reveals chromosomal gene expression regulation. Nature methods. 2013 Mar;10(3):246-8