Improved protocol for recovery of bacterial DNA from water filters: Sonication and backflushing of commercial syringe filters.

Extraction of bacterial DNA from water filters is frequently used in aquatic microbial ecology, e.g., for sequencing-based diversity studies. Here, we describe an improved size-class filtration and bacterial DNA extraction protocol using commercial "syringe filters", involving sonication- and backflushing-steps and DNA-binding magnetic beads. Importantly, the sonication-step improved DNA recovery up to 10-fold. The DNA extraction-turnaround time is 4h, with a hands-on time of about 2h. Copyright © 2013 Elsevier B.V. All rights reserved.

Citation

Anna Isabella Kesberg, David Schleheck. Improved protocol for recovery of bacterial DNA from water filters: Sonication and backflushing of commercial syringe filters. Journal of microbiological methods. 2013 Apr;93(1):55-7

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PMID: 23419826

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