Anna Isabella Kesberg, David Schleheck
Department of Biology, University of Konstanz,Konstanz, Germany.
Journal of microbiological methods 2013 AprExtraction of bacterial DNA from water filters is frequently used in aquatic microbial ecology, e.g., for sequencing-based diversity studies. Here, we describe an improved size-class filtration and bacterial DNA extraction protocol using commercial "syringe filters", involving sonication- and backflushing-steps and DNA-binding magnetic beads. Importantly, the sonication-step improved DNA recovery up to 10-fold. The DNA extraction-turnaround time is 4h, with a hands-on time of about 2h. Copyright © 2013 Elsevier B.V. All rights reserved.
Anna Isabella Kesberg, David Schleheck. Improved protocol for recovery of bacterial DNA from water filters: Sonication and backflushing of commercial syringe filters. Journal of microbiological methods. 2013 Apr;93(1):55-7
PMID: 23419826
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