Reconstructing evolutionary adaptive paths for protein engineering.

Reconstructing Evolutionary Adaptive Paths (REAP) is one of several methods to improve enzyme -functionality. This approach incorporates computational and theoretical aspects of protein engineering to create a focused library of protein variety with a high degree of functionality. In contrast to other -techniques like DNA shuffling, REAP allows a library to have diverse functionality among relatively few variants. REAP is a low-throughput method which takes advantage of natural selection and uses ancestral protein sequences to direct gene mutations, thereby creating a library with a high density of viable proteins. These proteins must then be assayed to characterize their functionality to identify which variants have the desired traits such as acid stability or thermostability.

Citation

Megan F Cole, Vanessa E Cox, Kelsey L Gratton, Eric A Gaucher. Reconstructing evolutionary adaptive paths for protein engineering. Methods in molecular biology (Clifton, N.J.). 2013;978:115-25

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PMID: 23423892

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