Preparation of cell lines for single-cell analysis of transcriptional activation dynamics.

Ilona U Rafalska-Metcalf, Susan M Janicki

Methods in molecular biology (Clifton, N.J.) 2013

filter terms:

Imaging molecularly defined regions of chromatin in single living cells during transcriptional activation has the potential to provide new insight into gene regulatory mechanisms. Here, we describe a method for isolating cell lines with multi-copy arrays of reporter transgenes, which can be used for real-time high-resolution imaging of transcriptional activation dynamics in single cells.

Citation

Ilona U Rafalska-Metcalf, Susan M Janicki. Preparation of cell lines for single-cell analysis of transcriptional activation dynamics. Methods in molecular biology (Clifton, N.J.). 2013;977:249-58