Construction of an infectious cDNA clone of radish mosaic virus, a crucifer-infecting comovirus.

Ken Komatsu, Masayoshi Hashimoto, Yukari Okano, Takuya Keima, Yugo Kitazawa, Takamichi Nijo, Shuichiro Takahashi, Kensaku Maejima, Yasuyuki Yamaji, Shigetou Namba

Laboratory of Plant Pathology, Department of Agricultural and Environmental Biology, Graduate School of Agricultural and Life Sciences, The University of Tokyo, 1-1-1 Yayoi, Bunkyo-ku, Tokyo, Japan.

Archives of virology 2013 Jul

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Radish mosaic virus (RaMV) is a crucifer-infecting comovirus that has been detected worldwide. Here, we report the successful construction of a full-length infectious cDNA clone of RaMV. The full-length cDNA clones corresponding to RNA1 and RNA2 of a Japanese isolate of RaMV were cloned into the pBlueScript plasmid or the binary vector pCAMBIA1301 downstream of the cauliflower mosaic virus 35S promoter. Mechanical inoculation or agroinoculation of Nicotiana benthamiana with these vectors resulted in systemic RaMV infections causing symptoms similar to those caused by the wild-type parental virus. The presence of progeny virus was verified by western blot analysis and electron microscopy.

Citation

Ken Komatsu, Masayoshi Hashimoto, Yukari Okano, Takuya Keima, Yugo Kitazawa, Takamichi Nijo, Shuichiro Takahashi, Kensaku Maejima, Yasuyuki Yamaji, Shigetou Namba. Construction of an infectious cDNA clone of radish mosaic virus, a crucifer-infecting comovirus. Archives of virology. 2013 Jul;158(7):1579-82