A role for the nucleoporin Nup170p in chromatin structure and gene silencing.

David W Van de Vosse, Yakun Wan, Diego L Lapetina, Wei-Ming Chen, Jung-Hsien Chiang, John D Aitchison, Richard W Wozniak

Cell 2013 Feb 28

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Embedded in the nuclear envelope, nuclear pore complexes (NPCs) not only regulate nuclear transport but also interface with transcriptionally active euchromatin, largely silenced heterochromatin, as well as the boundaries between these regions. It is unclear what functional role NPCs play in establishing or maintaining these distinct chromatin domains. We report that the yeast NPC protein Nup170p interacts with regions of the genome that contain ribosomal protein and subtelomeric genes, where it functions in nucleosome positioning and as a repressor of transcription. We show that the role of Nup170p in subtelomeric gene silencing is linked to its association with the RSC chromatin-remodeling complex and the silencing factor Sir4p, and that the binding of Nup170p and Sir4p to subtelomeric chromatin is cooperative and necessary for the association of telomeres with the nuclear envelope. Our results establish the NPC as an active participant in silencing and the formation of peripheral heterochromatin. Copyright © 2013 Elsevier Inc. All rights reserved.

Citation

David W Van de Vosse, Yakun Wan, Diego L Lapetina, Wei-Ming Chen, Jung-Hsien Chiang, John D Aitchison, Richard W Wozniak. A role for the nucleoporin Nup170p in chromatin structure and gene silencing. Cell. 2013 Feb 28;152(5):969-83