Krüppel-like factor 12 negatively regulates human endometrial stromal cell decidualization.

Xiaoyue Shen, Yali Hu, Yue Jiang, Hongyu Liu, Lihua Zhu, Xiaoyan Jin, Huizhi Shan, Xin Zhen, Lihua Sun, Guijun Yan, Haixiang Sun

Reproductive Medicine Center, the Affiliated Drum Tower Hospital of Nanjing University Medical School, Nanjing 210008, People's Republic of China.

Biochemical and biophysical research communications 2013 Mar 29

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Members of the KLFs family of transcription factors play roles in maternal endometrium development during embryo implantation. However, the specific role of KLF12 in endometrium development has not yet been described. In this study, we showed that KLF12 expression in human endometrial stromal cells (HESCs) was significantly decreased after decidualization stimulated by 8-Br-cAMP and medroxyprogesterone acetate (MPA). The adenovirus-mediated overexpression of KLF12 in HESCs significantly repressed the expression and secretion of decidualization biomarker genes and their products decidual prolactin (PRL) and insulin-like growth factor binding protein-1 (IGFBP-1) induced by 8-Br-cAMP and MPA. Moreover, CHIP and luciferase reporter assays demonstrated that KLF12 bound to a CAGTGGG element within the decidual prolactin promoter and decreased decidual PRL promoter (dPRL/-2000Luc) activation in a sequence-specific manner. Taken together, these findings suggest KLF12 is a negative regulator of human endometrial stromal cell decidualization. Crown Copyright © 2013. Published by Elsevier Inc. All rights reserved.

Citation

Xiaoyue Shen, Yali Hu, Yue Jiang, Hongyu Liu, Lihua Zhu, Xiaoyan Jin, Huizhi Shan, Xin Zhen, Lihua Sun, Guijun Yan, Haixiang Sun. Krüppel-like factor 12 negatively regulates human endometrial stromal cell decidualization. Biochemical and biophysical research communications. 2013 Mar 29;433(1):11-7