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Glutaraldehyde causes especially high autofluorescence. It reacted with proteins and peptides to generate visible to near-IR emitters. A model indicated that ethylenediamine and a secondary amine in the molecule were key components for the formation of emissive species. The mechanism enables us to control the generation and elimination of autofluorescence.


Kwahun Lee, Sungmoon Choi, Chun Yang, Hai-Chen Wu, Junhua Yu. Autofluorescence generation and elimination: a lesson from glutaraldehyde. Chemical communications (Cambridge, England). 2013 Apr 14;49(29):3028-30

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PMID: 23462727

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