Autofluorescence generation and elimination: a lesson from glutaraldehyde.

Kwahun Lee, Sungmoon Choi, Chun Yang, Hai-Chen Wu, Junhua Yu

Department of Chemistry Education, Seoul National University, 1 Gwanak-Ro, Gwanak-Gu, Seoul 151-742, South Korea.

Chemical communications (Cambridge, England) 2013 Apr 14

filter terms:

Glutaraldehyde causes especially high autofluorescence. It reacted with proteins and peptides to generate visible to near-IR emitters. A model indicated that ethylenediamine and a secondary amine in the molecule were key components for the formation of emissive species. The mechanism enables us to control the generation and elimination of autofluorescence.

Citation

Kwahun Lee, Sungmoon Choi, Chun Yang, Hai-Chen Wu, Junhua Yu. Autofluorescence generation and elimination: a lesson from glutaraldehyde. Chemical communications (Cambridge, England). 2013 Apr 14;49(29):3028-30