A new generation of food-borne pathogen detection based on ribosomal RNA.

Kristin Livezey, Shannon Kaplan, Michele Wisniewski, Michael M Becker

Roka Bioscience Inc., San Diego, CA, USA. klivezey@rokabio.com

Annual review of food science and technology 2013

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Listeria and Salmonella detection assays for food and environmental surfaces that target ribosomal RNA (rRNA) have been developed. The large number of rRNA molecules in bacteria enabled the development of molecular assays that use enrichment times as short as 12 hours for Salmonella and 24 hours for Listeria. These assays run on a fully automated molecular pathogen detection system, which provides walk-away capability and produces 300 assay results in eight hours.

Citation

Kristin Livezey, Shannon Kaplan, Michele Wisniewski, Michael M Becker. A new generation of food-borne pathogen detection based on ribosomal RNA. Annual review of food science and technology. 2013;4:313-25