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The development of synthetic biology requires rapid batch construction of large gene networks from combinations of smaller units. Despite the availability of computational predictions for well-characterized enzymes, the optimization of most synthetic biology projects requires combinational constructions and tests. A new building-brick-style parallel DNA assembly framework for simple and flexible batch construction is presented here. It is based on robust recombination steps and allows a variety of DNA assembly techniques to be organized for complex constructions (with or without scars). The assembly of five DNA fragments into a host genome was performed as an experimental demonstration.

Citation

Zhenyu Shi, Anthony G Wedd, Sally L Gras. Parallel in vivo DNA assembly by recombination: experimental demonstration and theoretical approaches. PloS one. 2013;8(2):e56854

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PMID: 23468883

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