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Capillary electrophoresis (CE) coupled with capacitively-coupled contactless conductivity (C(4)D) and fluorescence (FD) detectors and chip-CE for monitoring of nicotine and cotinine derivatization was demonstrated. Separation of the substrates and intermediates could be achieved by CE-C(4)D in 7 min (R(s) > 2.7) using 45 mM acetic acid (pH 3.0) and this system was applied to detect the intermediate formation. Final fluorescent products could be analyzed by micellar electrokinetic chromatography (MEKC-FD) in 5 mM borate buffer (pH 9.0) containing 10 mM sodium dodecylsulfate (SDS) (%RSD < 3.00%). Transferring of MEKC-FD to chip-CE allowed for shorter analysis time (2.5 min) and decreased sample consumption. The chip-CE-FD shows good detection and quantitation limits (< 7.5 ┬ÁM) and precision (%RSD < 2.81%) and was employed to determine nicotine and cotinine in artificial urine. This work reveals the potential of CE and chip-CE with dual detectors as simultaneous, convenient and rapid methods for monitoring pyridine derivatization.


Nantana Nuchtavorn, Marketa Ryvolova, Fritz Bek, Mirek Macka, Chutima Phechkrajang, Leena Suntornsuk. Potential of capillary electrophoresis (CE) and chip-CE with dual detection (capacitively-coupled contactless conductivity detection (C4D) and fluorescence detection) for monitoring of nicotine and cotinine derivatization. Analytical sciences : the international journal of the Japan Society for Analytical Chemistry. 2013;29(3):339-44

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PMID: 23474724

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