Engineering of therapeutic and diagnostic O-glycans on recombinant mucin-type immunoglobulin fusion proteins expressed in CHO cells.

Linda Lindberg, Jining Liu, Jan Holgersson

AbSorber AB, Stockholm, Sweden.

Methods in molecular biology (Clifton, N.J.) 2013

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Metabolic engineering of mammalian cells for optimized glycosylation is usually done to improve activity and the pharmacokinetic features of glycoprotein therapeutics. The field is mainly focused around engineering of N-glycans. We have created a platform in which recombinant mucin-type immu noglobulin fusion proteins are used as scaffolds for multivalent expression of O-glycans with diagnostic or therapeutic potential. The methods used to make stable CHO cell lines secreting a mucin-type fusion protein with blood group A or B determinants following expression of up to five different cDNAs are described.

Citation

Linda Lindberg, Jining Liu, Jan Holgersson. Engineering of therapeutic and diagnostic O-glycans on recombinant mucin-type immunoglobulin fusion proteins expressed in CHO cells. Methods in molecular biology (Clifton, N.J.). 2013;988:3-17