Multiplex polymerase chain reaction assay for the detection of minute virus of mice and mouse parvovirus infections in laboratory mice.

K W Wang, L L Chueh, M H Wang, Y T Huang, B H Fang, C Y Chang, M C Fang, J Y Chou, S C Hsieh, C H Wan

Graduate Institute of Veterinary Medicine, National Taiwan University, Taipei, Taiwan, ROC.

Laboratory animals 2013 Apr

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Mouse parvoviruses are among the most prevalent infectious pathogens in contemporary mouse colonies. To improve the efficiency of routine screening for mouse parvovirus infections, a multiplex polymerase chain reaction (PCR) assay targeting the VP gene was developed. The assay detected minute virus of mice (MVM), mouse parvovirus (MPV) and a mouse housekeeping gene (α-actin) and was able to specifically detect MVM and MPV at levels as low as 50 copies. Co-infection with the two viruses with up to 200-fold differences in viral concentrations can easily be detected. The multiplex PCR assay developed here could be a useful tool for monitoring mouse health and the viral contamination of biological materials.

Citation

K W Wang, L L Chueh, M H Wang, Y T Huang, B H Fang, C Y Chang, M C Fang, J Y Chou, S C Hsieh, C H Wan. Multiplex polymerase chain reaction assay for the detection of minute virus of mice and mouse parvovirus infections in laboratory mice. Laboratory animals. 2013 Apr;47(2):116-21