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A simple assay for the ribonuclease activity of ribonucleases in the presence of ethidium bromide.

Debi Ranjan Tripathy, Amit Kumar Dinda, Swagata Dasgupta

Department of Chemistry, Indian Institute of Technology, Kharagpur, Kharagpur 721302, India.

Analytical biochemistry 2013 Jun 15


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  • active site (1)
  • angiogenin (1)
  • binding (2)
  • change intensity (2)
  • degradation rna (1)
  • ethidium bromide (3)
  • fluorescence (2)
  • ribonuclease (4)
  • ribonuclease activity (2)
  • rna (3)
  • RNase A (2)
  • the change (2)
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    The ribonuclease (RNase) activity of ribonucleases has been assayed by observing the change in fluorescence intensity of ethidium bromide on binding with yeast RNA. The binding of EtBr with RNA was monitored via UV-vis and fluorimetric methods. The degradation of RNA by RNase A was monitored by the change in fluorescence emission intensity of ethidium bromide at 600nm on excitation at 510nm. The ribonucleolytic activity of RNase A and angiogenin at various pH values was determined by this method. From this technique we have also determined the macroscopic pKa values of active site residues of these enzymes. This assay permits the evaluation of the catalytic efficiency of enzymatic proteins ranging from high ribonucleolytic activity to low ribonucleolytic activity toward the natural substrate RNA. Copyright © 2013 Elsevier Inc. All rights reserved.

    Citation

    Debi Ranjan Tripathy, Amit Kumar Dinda, Swagata Dasgupta. A simple assay for the ribonuclease activity of ribonucleases in the presence of ethidium bromide. Analytical biochemistry. 2013 Jun 15;437(2):126-9


    PMID: 23499964

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