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Currently protease assay kits, requiring substrate that is either radiolabeled or fluorescence labeled and specialized instruments, are all expensive. A simple, reliable assay of protease activity and its inhibitor screening for general laboratory is rare. Here we demonstrated a straightforward ninhydrin-based method for assay of collagenase activity and its inhibitor screening using spectrophotometry. In the method, without multistep sample treatments and substrate labeling, the hydrolytic products were directly traced by ninhydrin. The method is expected to be suitable for not only the assay of collagenase activity but also the others matrix metalloproteinases activities, and can be used for kinetic study. Copyright © 2013 Elsevier Inc. All rights reserved.


Yanfang Zhang, Yun Fu, Sufeng Zhou, Lixia Kang, Changzheng Li. A straightforward ninhydrin-based method for collagenase activity and inhibitor screening of collagenase using spectrophotometry. Analytical biochemistry. 2013 Jun 1;437(1):46-8

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PMID: 23499966

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