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Plant and animal genomes are replete with large gene families, making the task of ortholog identification difficult and labor intensive. OrthoRBH is an automated reciprocal blast pipeline tool enabling the rapid identification of specific gene families of interest in related species, streamlining the collection of homologs prior to downstream molecular evolutionary analysis. The efficacy of OrthoRBH is demonstrated with the identification of the 13-member PYR/PYL/RCAR gene family in Hordeum vulgare using Oryza sativa query sequences. OrthoRBH runs on the Linux command line and is freely available at SourceForge. orthorbh


Mark Ziemann, Atul Kamboj, Mrinal Bhave. OrthoRBH: A streamlined pipeline for mining large gene family sequences in related species. Bioinformation. 2013;9(5):267-9

PMID: 23515897

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