Cap1p attenuates the apoptosis of Candida albicans.

Bao-Di Dai, Yan Wang, Lan-Xue Zhao, De-Dong Li, Ming-Bang Li, Yong-Bing Cao, Yuan-Ying Jiang

New Drug Research and Development Center, School of Pharmacy, Second Military Medical University, Shanghai 200433, China.

The FEBS journal 2013 Jun

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Candida albicans is the most common opportunistic fungal pathogen and its apoptosis is inducible by environmental stress. Based on our previous finding that transcription factor Cap1p was involved in baicalein-induced apoptosis, the present study aimed to further clarify the role of Cap1p in apoptosis by observing the impact of CAP1 deletion on cell fate. It was found that apoptotic stimulation with amphotericin B, acetic acid and hydrogen peroxide increased the number of apoptotic and necrotic cells, caspase activity and the accumulation of reactive oxygen species, whereas it decreased the mitochondrial membrane potential and intracellular ATP level in the cap1Δ/Δ mutant. The cell fate was, at least partly, caused by glutathione depletion and attenuation of the expression of the glutathione reductase gene in the cap1Δ/Δ mutant. Collectively, our data suggest that Cap1p participated in the apoptosis of C. albicans by regulating the expression of the glutathione reductase gene and glutathione content. © 2013 The Authors Journal compilation © 2013 FEBS.

Citation

Bao-Di Dai, Yan Wang, Lan-Xue Zhao, De-Dong Li, Ming-Bang Li, Yong-Bing Cao, Yuan-Ying Jiang. Cap1p attenuates the apoptosis of Candida albicans. The FEBS journal. 2013 Jun;280(11):2633-43