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We report a monomeric yellow-green fluorescent protein, mNeonGreen, derived from a tetrameric fluorescent protein from the cephalochordate Branchiostoma lanceolatum. mNeonGreen is the brightest monomeric green or yellow fluorescent protein yet described to our knowledge, performs exceptionally well as a fusion tag for traditional imaging as well as stochastic single-molecule superresolution imaging and is an excellent fluorescence resonance energy transfer (FRET) acceptor for the newest cyan fluorescent proteins.


Nathan C Shaner, Gerard G Lambert, Andrew Chammas, Yuhui Ni, Paula J Cranfill, Michelle A Baird, Brittney R Sell, John R Allen, Richard N Day, Maria Israelsson, Michael W Davidson, Jiwu Wang. A bright monomeric green fluorescent protein derived from Branchiostoma lanceolatum. Nature methods. 2013 May;10(5):407-9

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PMID: 23524392

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