Effect of PSI-697, a novel P-selectin inhibitor, on platelet-monocyte aggregate formation in humans.

Alan G Japp, Raj Chelliah, Laura Tattersall, Ninian N Lang, Xu Meng, Kathleen Weisel, Arie Katz, David Burt, Keith A A Fox, Giora Z Feuerstein, Thomas M Connolly, David E Newby

Journal of the American Heart Association 2013 Jan 28

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Platelet activation is central to the pathogenesis of acute coronary syndromes. Surface expression of P-selectin on activated platelets induces formation of platelet-monocyte aggregates and promotes vascular inflammation and thrombosis. P-selectin antagonism may represent a novel therapeutic strategy in vascular disease. We aimed to investigate the effects of the novel P-selectin antagonist PSI-697 on platelet-monocyte aggregate formation in humans. In a double-blind, randomized, placebo-controlled crossover study, healthy smokers were randomized to receive either oral PSI-697 600 mg or matched placebo. The sequence of treatment was also randomized, with all subjects receiving both PSI-697 and placebo. Platelet-monocyte aggregates were measured by flow cytometry at 4 and 24 hours in the presence and absence of thrombin receptor-activating peptide (TRAP; 0.1 to 1.0 μm/L). The ex vivo addition of TRAP caused a concentration-dependent increase in platelet-monocyte aggregates from 8.2% to 94.8% (P<0.001). At 4 and 24 hours, plasma concentrations of PSI-697 increased to 1906 and 83 ng/mL, respectively (P<0.001). PSI-697 had no demonstrable effect on either stimulated or unstimulated platelet-monocyte aggregates at 4 or 24 hours (P>0.05). P-selectin-blocking antibody (CLB-Thromb6), but not PSI-697, inhibited both stimulated and unstimulated platelet-monocyte aggregate formation in vitro (P<0.001). The novel small-molecule P-selectin antagonist PSI-697 did not inhibit basal or stimulated platelet-monocyte aggregate formation in humans at the dose tested. Its clinical efficacy remains to be established. URL: http://EudraCT.ema.europa.eu Unique identifier: 2007-005695-14.

Citation

Alan G Japp, Raj Chelliah, Laura Tattersall, Ninian N Lang, Xu Meng, Kathleen Weisel, Arie Katz, David Burt, Keith A A Fox, Giora Z Feuerstein, Thomas M Connolly, David E Newby. Effect of PSI-697, a novel P-selectin inhibitor, on platelet-monocyte aggregate formation in humans. Journal of the American Heart Association. 2013 Jan 28;2(1):e006007