Approaches to cloning of pain-related ion channel genes.

Department of Endodontics, Dental School, University of Texas Health Science Center at San Antonio, San Antonio, TX, USA.

Methods in molecular biology (Clifton, N.J.) 2013

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Molecular pain research is a relatively new and rapidly expanding field that represents advancement in conventional pain research. One of the fundamentals of molecular pain involves the clon ing of genes and especially the ion channels specifically involved in nociceptive processing at the periphery and centrally. A variety of approaches were used to isolate these critically important genes. Cloning of these genes involved innovative strategies based on existing molecular approaches. This review will discuss well-utilized cloning approaches and their exploitation in molecular pain research.