AlphaLISA for the determination of median levels of the free β subunit of human chorionic gonadotropin in the serum of pregnant women.

Li-Ping Zou, Tian-Cai Liu, Guan-Feng Lin, Zhi-Ning Dong, Jing-Yuan Hou, Ming Li, Ying-Song Wu

Institute of Antibody Engineering, School of Biotechnology, Southern Medical University, Guangzhou, China.

Journal of immunoassay & immunochemistry 2013

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Measurement of the free β subunit of human chorionic gonadotropin (free β-hCG) in serum is useful for prenatal screening. Concentrations of free β-hCG vary in different races. Conventional assays used for such measurements have limitations. We applied the AlphaLISA to measure levels of free β-hCG in maternal serum during 8-20 weeks of gestation in women from southern China. Two anti-free β-hCG antibodies were used: one was coated on AlphaLISA acceptor beads and the one was biotinylated. The assay also contained donor beads coated with streptavidin. The AlphaLISA assay detection limit was 0.11 ng/mL, and the analytical range was 0.11-200 ng/mL. The intra- and interassay coefficients of variation were 1.32%-2.50% and 3.44%-5.45%, respectively. The correlation with commercial Eu(3+)-labeled free β-HCG-TRFIA assay was good (y = 1.045x + 1.580, r(2) = 0.978). Median levels of free β-hCG in maternal serum at 8-20 weeks gestation were higher in women from southern China compared with those reported in women from other countries. The AlphaLISA for free β-HCG could become the assay of choice for applications in clinical diagnostics. The established median value of free β-HCG is helpful in clinical diagnosis specific for southern Chinese women.

Citation

Li-Ping Zou, Tian-Cai Liu, Guan-Feng Lin, Zhi-Ning Dong, Jing-Yuan Hou, Ming Li, Ying-Song Wu. AlphaLISA for the determination of median levels of the free β subunit of human chorionic gonadotropin in the serum of pregnant women. Journal of immunoassay & immunochemistry. 2013;34(2):134-48