Hanane Messiad, Habiba Amira-Guebailia, Omar Houache
Laboratory of Applied Chemistry, 8 Mai 1945 Guelma University, Guelma 24000, Algeria.
Journal of chromatography. B, Analytical technologies in the biomedical and life sciences 2013 May 1Piceatannol is one of resveratrol derivatives having health promoting potential. However, its low water-solubility and bioavailability could limit its use in both food and pharmaceutical fields. The aim of this work is the study of piceatannol complexation by β-cyclodextrin (β-CD) in aqueous media. The complex formed could improve the bioavailability, the solubility and the stability of piceatannol. The method used was based on RP-HPLC in which, β-CD was added to methanol/water mixtures mobile phases. The apparent formation constant of piceatannol/β-cyclodextrin complex was determined. Within the concentration interval studied (0-4 mM), whenever the concentration of β-CD increased, retention time of piceatannol decreased; indicating an enhancement of solubility due to the formation of the piceatannol/β-CD complex. The formation constants (KF) of the piceatannol/β-CD complex varied significantly with both the methanol concentration in the water-methanol mixture and the temperature. Moreover, in all the physicochemical conditions tested, the stoichiometry of piceatannol/β-CD complex was 1:1. Finally, the thermodynamic parameters were determined: ΔG°=-13.123 kJ mol(-1), ΔH°=-33. 265 kJ mol(-1) and ΔS°=-67.559 J mol(-1) K(-1). Copyright © 2013 Elsevier B.V. All rights reserved.
Hanane Messiad, Habiba Amira-Guebailia, Omar Houache. Reversed phase High Performance Liquid Chromatography used for the physicochemical and thermodynamic characterization of piceatannol/β-cyclodextrin complex. Journal of chromatography. B, Analytical technologies in the biomedical and life sciences. 2013 May 1;926:21-7
PMID: 23537743
View Full Text