Evaluation of tropicamide-loaded tamarind seed xyloglucan nanoaggregates for ophthalmic delivery.

Neeraj Dilbaghi, Harmanmeet Kaur, Munish Ahuja, Sandeep Kumar

Nanomaterials Synthesis & Characterization Laboratory, Department of Bio & Nano Technology, Guru Jambheshwar University of Science & Technology, Hisar 125001, India.

Carbohydrate polymers 2013 Apr 15

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The present study was aimed to prepare tamarind seed nanoaggregates and its evaluation for ophthalmic delivery. The preparation of tropicamide-loaded tamarind seed xyloglucan nanoaggregates was optimized using face centred central composite experimental design, employing the concentrations of tamarind seed xyloglucan and Poloxamer-407, as independent variables. The results revealed that concentration of TSX has a significant antagonistic effect on particle size, while poloxamer displayed a significant synergistic effect on encapsulation efficiency. The optimal concentrations of TSX and poloxamer were found to be 0.45% (w/v) and 0.5% (w/v) respectively. The optimized formulation of tropicamide-loaded TSX nanoaggregates showed a significantly higher corneal permeation of tropicamide across the isolated goat cornea compared to commercial conventional aqueous formulation. The results revealed excellent mucoadhesive properties of TSX nanoaggregates. Further, the tropicamide-loaded TSX nanoaggregates formulation showed excellent ocular tolerance and biocompatibility as determined by hen's egg test chorioallantoic membrane and resazurin assay on Vero cell lines. Copyright © 2013 Elsevier Ltd. All rights reserved.

Citation

Neeraj Dilbaghi, Harmanmeet Kaur, Munish Ahuja, Sandeep Kumar. Evaluation of tropicamide-loaded tamarind seed xyloglucan nanoaggregates for ophthalmic delivery. Carbohydrate polymers. 2013 Apr 15;94(1):286-91