A label-free supersandwich electrogenerated chemiluminescence method for the detection of DNA methylation and assay of the methyltransferase activity.

Yan Li, Xiaoe Luo, Zhao Yan, Jianbin Zheng, Honglan Qi

Key Laboratory of Electroanalytical Chemistry, Institute of Analytical Science, Northwest University, Xi'an, Shaanxi 710069, PR China. yanli@nwu.edu.cn

Chemical communications (Cambridge, England) 2013 May 10

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A method based on electrogenerated chemiluminescence (ECL) for detection of DNA methylation and assay of the methyltransferase activity is developed, and it is demonstrated that the label-free ECL method is capable of detecting methyltransferase with a detection limit of 3 × 10(-6) U mL(-1), using a supersandwich amplification technique.

Citation

Yan Li, Xiaoe Luo, Zhao Yan, Jianbin Zheng, Honglan Qi. A label-free supersandwich electrogenerated chemiluminescence method for the detection of DNA methylation and assay of the methyltransferase activity. Chemical communications (Cambridge, England). 2013 May 10;49(37):3869-71