Interactions of nanoparticles with proteins: determination of equilibrium constants.
Lennart Treuel, Marcelina Malissek
Institute of Applied Physics and Center for Functional Nanostructures (CFN), Karlsruhe Institute of Technology (KIT), Karlsruhe, Germany.
Methods in molecular biology (Clifton, N.J.) 2013The behavior of nanoparticles towards proteins is an important aspect across wide areas of nanotoxicology and nanomedicine. In this chapter, we describe a procedure to study the adsorption of proteins onto nanoparticle surfaces. Circular dichroism (CD) spectroscopy is utilized to quantify the amount of free protein in a solution, and the experimental information is evaluated to derive equilibrium constants for the protein adsorption/desorption equilibrium. These equilibrium constants are comparable parameters in describing the interactions between proteins and nanoparticles.
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Lennart Treuel, Marcelina Malissek. Interactions of nanoparticles with proteins: determination of equilibrium constants. Methods in molecular biology (Clifton, N.J.). 2013;991:225-35
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PMID: 23546673
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