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The behavior of nanoparticles towards proteins is an important aspect across wide areas of nanotoxicology and nanomedicine. In this chapter, we describe a procedure to study the adsorption of proteins onto nanoparticle surfaces. Circular dichroism (CD) spectroscopy is utilized to quantify the amount of free protein in a solution, and the experimental information is evaluated to derive equilibrium constants for the protein adsorption/desorption equilibrium. These equilibrium constants are comparable parameters in describing the interactions between proteins and nanoparticles.

Citation

Lennart Treuel, Marcelina Malissek. Interactions of nanoparticles with proteins: determination of equilibrium constants. Methods in molecular biology (Clifton, N.J.). 2013;991:225-35

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PMID: 23546673

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