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Human embryonic stem cells can be cultured and maintained on fibroblast feeder cells of murine or human origin. Thorough protocols are provided for the growth and maintenance of human embryonic stem cells on either feeder cell type.

Citation

Jasmeet Kaur, Mary Lynn Tilkins. Methods for culturing human embryonic stem cells on feeders. Methods in molecular biology (Clifton, N.J.). 2013;997:93-113

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PMID: 23546750

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